48-well devices cytoview mea plate Search Results


24 well cytoview mea plates m384-tmea-24w  (Axion BioSystems)
90
Axion BioSystems 24 well cytoview mea plates m384-tmea-24w
24 Well Cytoview Mea Plates M384 Tmea 24w, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+devices+cytoview+mea+plate/cytoview+48+well+mea+plates/pmc10168378-236-2-5
Average 90 stars, based on 1 article reviews
24 well cytoview mea plates m384-tmea-24w - by Bioz Stars, 2026-11
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cytoview mea plates 48-well  (Axion BioSystems)
90
Axion BioSystems cytoview mea plates 48-well
All experiments were undertaken in DIV12 cultures cultured in <t>CytoView</t> micro-electrode array <t>(MEA)</t> multi-well plates using the Maestro-Pro MEA system (Axion Biosystems). A Each well contains 16 electrodes that can record local activity B Fluorescent microscopy shows DIV12 primary cortical neurons transduced with AAV-Syn1-eGFP and cultured on a micro-electrode plate. Scale bar = 275 µm. C An example of a raster plot, which shows firing over time, taken at baseline from untransduced neurons. Each row corresponds to an individual electrode. Action potentials are recorded as spikes (black lines) and this is termed ‘firing’. A burst (blue) occurs when multiples action potentials fire in rapid succession in the locality of an electrode; a minimum threshold is set at 50 spikes within 100 ms. A network burst was classified as at least 35% of electrodes recording bursts simultaneously; this shows that the neurons throughout the culture are connected. D Representative raster plots showing 60 s recordings of untransduced neurons at baseline and following treatment with 0.625 µM 4AP/12.5 µM Bicuculline (Bic). Baseline recordings show spontaneous firing and bursting. Treatment with 4AP/Bic stimulates synchronous and oscillatory firing across the well, as shown by a switch to network bursting. E 4AP/Bic treatment significantly increases activity and ( F ) burst strength ( t -test, **** P < 0.0001, n = 3 technical repeats). G Representative raster plots comparing neurons overexpressing eGFP, eGFP-Tau WT or eGFP-Tau P301L at baseline and following treatment with 4AP/Bic. H At baseline, the activity and ( I ) burst strength of neurons overexpressing eGFP-Tau P301L is significantly greater than those expressing eGFP or eGFP-Tau WT . Following treatment with 4AP/Bic, activity but not burst strength is significantly greater with eGFP-Tau P301L than eGFP or eGFP-Tau WT (two-way ANOVA with Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 4 technical repeats).
Cytoview Mea Plates 48 Well, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+devices+cytoview+mea+plate/cytoview+mea+plates+48+well/pmc11189525-96-3-6
Average 90 stars, based on 1 article reviews
cytoview mea plates 48-well - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

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All experiments were undertaken in DIV12 cultures cultured in CytoView micro-electrode array (MEA) multi-well plates using the Maestro-Pro MEA system (Axion Biosystems). A Each well contains 16 electrodes that can record local activity B Fluorescent microscopy shows DIV12 primary cortical neurons transduced with AAV-Syn1-eGFP and cultured on a micro-electrode plate. Scale bar = 275 µm. C An example of a raster plot, which shows firing over time, taken at baseline from untransduced neurons. Each row corresponds to an individual electrode. Action potentials are recorded as spikes (black lines) and this is termed ‘firing’. A burst (blue) occurs when multiples action potentials fire in rapid succession in the locality of an electrode; a minimum threshold is set at 50 spikes within 100 ms. A network burst was classified as at least 35% of electrodes recording bursts simultaneously; this shows that the neurons throughout the culture are connected. D Representative raster plots showing 60 s recordings of untransduced neurons at baseline and following treatment with 0.625 µM 4AP/12.5 µM Bicuculline (Bic). Baseline recordings show spontaneous firing and bursting. Treatment with 4AP/Bic stimulates synchronous and oscillatory firing across the well, as shown by a switch to network bursting. E 4AP/Bic treatment significantly increases activity and ( F ) burst strength ( t -test, **** P < 0.0001, n = 3 technical repeats). G Representative raster plots comparing neurons overexpressing eGFP, eGFP-Tau WT or eGFP-Tau P301L at baseline and following treatment with 4AP/Bic. H At baseline, the activity and ( I ) burst strength of neurons overexpressing eGFP-Tau P301L is significantly greater than those expressing eGFP or eGFP-Tau WT . Following treatment with 4AP/Bic, activity but not burst strength is significantly greater with eGFP-Tau P301L than eGFP or eGFP-Tau WT (two-way ANOVA with Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 4 technical repeats).

Journal: Cell Death & Disease

Article Title: Tau P301L disengages from the proteosome core complex and neurogranin coincident with enhanced neuronal network excitability

doi: 10.1038/s41419-024-06815-2

Figure Lengend Snippet: All experiments were undertaken in DIV12 cultures cultured in CytoView micro-electrode array (MEA) multi-well plates using the Maestro-Pro MEA system (Axion Biosystems). A Each well contains 16 electrodes that can record local activity B Fluorescent microscopy shows DIV12 primary cortical neurons transduced with AAV-Syn1-eGFP and cultured on a micro-electrode plate. Scale bar = 275 µm. C An example of a raster plot, which shows firing over time, taken at baseline from untransduced neurons. Each row corresponds to an individual electrode. Action potentials are recorded as spikes (black lines) and this is termed ‘firing’. A burst (blue) occurs when multiples action potentials fire in rapid succession in the locality of an electrode; a minimum threshold is set at 50 spikes within 100 ms. A network burst was classified as at least 35% of electrodes recording bursts simultaneously; this shows that the neurons throughout the culture are connected. D Representative raster plots showing 60 s recordings of untransduced neurons at baseline and following treatment with 0.625 µM 4AP/12.5 µM Bicuculline (Bic). Baseline recordings show spontaneous firing and bursting. Treatment with 4AP/Bic stimulates synchronous and oscillatory firing across the well, as shown by a switch to network bursting. E 4AP/Bic treatment significantly increases activity and ( F ) burst strength ( t -test, **** P < 0.0001, n = 3 technical repeats). G Representative raster plots comparing neurons overexpressing eGFP, eGFP-Tau WT or eGFP-Tau P301L at baseline and following treatment with 4AP/Bic. H At baseline, the activity and ( I ) burst strength of neurons overexpressing eGFP-Tau P301L is significantly greater than those expressing eGFP or eGFP-Tau WT . Following treatment with 4AP/Bic, activity but not burst strength is significantly greater with eGFP-Tau P301L than eGFP or eGFP-Tau WT (two-way ANOVA with Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 4 technical repeats).

Article Snippet: Twenty-four and 48-well CytoView MEA Plates (Axion Biosystems) were coated with 0.1% polyethylenimine (PEI) in borate buffer (100 mM Boric acid, 75 mM NaCl, 25 mM Sodium tetraborate, pH 8.4).

Techniques: Cell Culture, Activity Assay, Microscopy, Transduction, Expressing